Journal: Eye and Vision

Article Title: Ex vivo cultivated retinal pigment epithelial cell transplantation for the treatment of rabbit corneal endothelial dysfunction

doi: 10.1186/s40662-023-00351-4

Figure Lengend Snippet: Antibodies for immunofluorescence staining

Article Snippet: Mouse monoclonal to MITF , Abcam , ab3201 , 1:100.

Techniques: Immunofluorescence

Journal: PLoS ONE

Article Title: A Gene Expression Signature of Invasive Potential in Metastatic Melanoma Cells

doi: 10.1371/journal.pone.0008461

Figure Lengend Snippet: (A) Unsupervised hierarchical clustering by using 96 genes identified by class comparison as differentially expressed between relatively higher or lower MITF -expressing melanoma cell lines classified the cell lines into two main groups (Motif 1, green label; Motif 2, black label). (B) qPCR validation of selected targets in a subset of Motif 1 and 2 NZM cell lines. Unsupervised clustering of qPCR data confirmed the Motif 1 (green) and 2 (black) classifications. (C) Protein expression of MITF targets CDK2, BCL2, and MLANA agreed with MITF transcript levels and array and qPCR cell line stratification. *Motif 1 cell lines. (D) Immunofluorescence showed stronger staining for MITF and MLANA in the Motif 2 NZM06 cells compared to Motif 1 NZM09 cells.

Article Snippet: Coverslips were rinsed with PBS before incubation with 1% BSA in PBS for 30 min. to block non-specific antibody binding, then incubated for 60 min. at room temperature with either mouse monoclonal anti-MLANA antibody, Santa Cruz), or mouse monoclonal anti-MITF antibody (clone C5+D5, Zymed).

Techniques: Expressing, Immunofluorescence, Staining